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Updated: Jun 17, 2026
Monitoring Protein Adsorption with Solid-state Nanopores
Published on: December 2, 2011
Sensing of protein adsorption with a porous bulk composite comprising silver nanoparticles deposited on
Chikara Ohtsuki1, Yuji Ichikawa, Hiroyuki Shibata
1Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya, Japan. ohtsuki@apchem.nagoya-u.ac.jp
Abstract:
Porous bulk composites were produced by depositing silver nanoparticles of diameter 11.0 +/- 3.2 nm on hydroxyapatite of micrometer sizes. Adsorption of bovine serum albumin (BSA) and lysozyme (LSZ) on the composite material was observed in 2 and 10 mol m(-3) phosphate buffer solutions. More BSA than LSZ was adsorbed in 2 mol m(-3) phosphate buffer and this was attributed to a larger a-face surface area present in the plate- and rod-shaped hydroxyapatite compared with the c-face surface area. Peak shifts in localized surface plasmon resonance (LSPR) spectra were clearly related to adsorbed amounts of BSA and LSZ after exposure of the porous bulk composites to protein solutions. The sensing capability of the porous bulk composite results from changes in the dielectric constant of the surface fluid surrounding the silver nanoparticles. Adsorption/desorption cycles of BSA were applied to the porous bulk composite, confirming the reversibility of the sensing capability.
