Related Experiment Video

Updated: Jun 17, 2026

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation
07:53

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Published on: January 9, 2019

Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia

Saeed Sharif1, Siti S Arshad, Mohd Hair-Bejo

  • 1Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

Acta Veterinaria Scandinavica
|January 8, 2010
PubMed

Insights

Feline coronaviruses (FCoVs) were detected in 89% of cats with feline infectious peritonitis (FIP) in Malaysia. Genetic analysis revealed Malaysian FCoV isolates form a distinct cluster, providing crucial insights into feline coronavirus in the region.

Area of Science:

  • Veterinary Virology
  • Molecular Epidemiology
  • Feline Infectious Diseases

Background:

  • Feline infectious peritonitis (FIP) is a severe disease in cats caused by feline coronaviruses (FCoVs).
  • Limited genetic data on FCoVs exists for Malaysia, hindering epidemiological understanding.

Purpose of the Study:

  • To investigate the distribution and genetic characteristics of FCoVs in cats with suspected FIP in Malaysia.
  • To provide the first genetic information on FCoV isolates from Malaysia.

Main Methods:

  • Reverse transcription polymerase chain reaction (RT-PCR) was used to detect FCoV in ascitic fluid and biopsy samples.
  • Nucleotide sequencing of the 3' untranslated region (3'UTR) of the FCoV genome was performed on positive samples.
  • Phylogenetic analysis was conducted to determine the genetic relationships of Malaysian FCoV isolates.

Main Results:

  • FCoV was detected in 89% of the sampled cats.
  • The majority of FCoV-positive cats were male (80%) and under 2 years old (64%).
  • Sequencing revealed Malaysian FCoV isolates share 96% homology with each other and 93% with reference strains, forming a distinct genetic cluster.

Conclusions:

  • Feline coronaviruses are highly prevalent in cats with FIP in Malaysia.
  • Malaysian FCoV isolates represent a genetically distinct group, contributing novel data to global FCoV phylogeny.
  • This study establishes a genetic baseline for FCoV in Malaysia, crucial for future disease control efforts.

More Related Videos

Standard Operating Procedure for Lyssavirus Surveillance of the Bat Population in Taiwan07:55

Standard Operating Procedure for Lyssavirus Surveillance of the Bat Population in Taiwan

Published on: August 27, 2019

Related Concept Videos

Poliomyelitis01:17

Poliomyelitis

Poliomyelitis is caused by poliovirus, a small, non-enveloped, positive-sense RNA virus of the Picornaviridae family and Enterovirus genus. Transmission occurs primarily via the fecal-oral route, often through ingestion of contaminated water or food. The virus initially replicates in the oropharynx and intestinal mucosa, particularly in lymphoid tissues such as the tonsils, Peyer’s patches, and regional lymph nodes. Primary viremia follows, allowing dissemination throughout the body.In most...
Toxoplasmosis01:28

Toxoplasmosis

Toxoplasmosis, a zoonotic disease caused by the protozoan Toxoplasma gondii, poses significant public health challenges globally due to its high seroprevalence and varied clinical manifestations. As an obligate intracellular parasite, T. gondii can infect all warm-blooded vertebrates, but felids are its only definitive hosts, shedding unsporulated oocysts into the environment. Humans typically acquire the infection through ingestion of tissue cysts in undercooked meat or oocysts from...