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Microassay for DNA methyltransferase
R L Adams1, A Rinaldi, C Seivwright
1Department of Biochemistry, University of Glasgow, UK.
Journal of Biochemical and Biophysical Methods
|January 1, 1991
Abstract:
A microassay for DNA methylase is described which can detect activity in as few as 50 tissue culture cells. The cells are lysed and incubated for 2 h at 37 degrees C with 3 microCi high specific activity [3H]AdoMet and 0.5 microgram poly[d(I-C).d(I-C)] in a volume of 23 microliters. Ribonuclease is present during the assay and the product DNA is isolated by phenol extraction after protease digestion.