Related Experiment Video

Updated: Jun 17, 2026

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

DOC45, a novel DNA damage-regulated nucleocytoplasmic ATPase that is overexpressed in multiple human malignancies

Hong Sun1, Xiuquan Luo, JoAnne Montalbano

  • 1Department of Pharmacology, State University of New York, Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA.

Molecular Cancer Research : MCR
|January 8, 2010
PubMed

Insights

We identified a new gene, DNA damage-regulated overexpressed in cancer 45 (DOC45), that is downregulated by DNA damage but overexpressed in many cancers. DOC45 is a novel ATPase involved in cell proliferation and survival.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Biochemistry

Background:

  • The cellular response to DNA damage involves complex regulatory networks.
  • Understanding novel genes involved in stress response and cancer is crucial for therapeutic development.

Purpose of the Study:

  • To characterize a novel gene, DNA damage-regulated overexpressed in cancer 45 (DOC45).
  • To investigate the role of DOC45 in DNA damage response, cancer development, and cellular processes.

Main Methods:

  • Gene expression analysis using DNA damage and endoplasmic reticulum stress agents.
  • Analysis of DOC45 expression in human malignancies.
  • Biochemical assays to determine ATPase activity.
  • Cellular localization studies and nucleocytoplasmic transport inhibition.
  • Gene knockdown experiments using short hairpin RNA (shRNA) in colon cancer cells.

Main Results:

  • DOC45 expression is strongly downregulated by DNA-damaging agents but not ER stress agents.
  • DOC45 is overexpressed in multiple human cancers (colon, rectum, ovary, lung, stomach, uterus).
  • DOC45 possesses nucleotide triphosphate-binding motifs and exhibits ATP hydrolysis activity, functioning as an ATPase.
  • DOC45 shows nucleocytoplasmic distribution and accumulates in nucleoli upon inhibition of nuclear export, suggesting shuttling.
  • DOC45 expression is upregulated during oncogenic Ras transformation and linked to the phosphoinositide 3-kinase pathway.
  • DOC45 knockdown inhibits colon cancer cell proliferation and increases sensitivity to doxorubicin-induced cell death.

Conclusions:

  • DOC45 is a novel ATPase implicated in cellular stress response and tumorigenesis.
  • DOC45 plays a significant role in cell proliferation and survival.
  • DOC45 may serve as a potential tumor marker for various human malignancies.

More Related Videos

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity04:20

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity

Published on: February 9, 2024

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...