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Heterokaryon Technique for Analysis of Cell Type-specific Localization
Published on: March 11, 2011
Heterodimerization with different Jun proteins controls c-Fos intranuclear dynamics and distribution.
Cécile E Malnou1, Frédérique Brockly, Cyril Favard
1Institut de Génétique Moléculaire de Montpellier, UMR5535, CNRS, 1919 route de Mende, 34293 Montpellier Cedex 5, France.
The Journal of Biological Chemistry
|January 8, 2010
Summary
The c-Fos transcription factor
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- c-Fos is a transcription factor regulating cellular and organismal processes.
- Its function requires heterodimerization with Jun proteins.
- Intranuclear mobility regulation of c-Fos is poorly understood.
Purpose of the Study:
- To investigate the intranuclear mobility and distribution of c-Fos.
- To determine how heterodimerization with Jun proteins affects c-Fos localization.
- To explore novel regulatory mechanisms of Fos protein function.
Main Methods:
- Fluorescence recovery after photobleaching.
- Genetic approaches.
- Biochemical approaches.
Main Results:
- Monomeric c-Fos is mobile and evenly distributed (excluding nucleoli).
- Heterodimerization with c-Jun dramatically reduces c-Fos mobility and alters distribution via nuclear matrix association, independent of DNA binding.
- Dimerization with JunB does not significantly alter c-Fos mobility but changes its intranuclear distribution.
Conclusions:
- Jun partners differentially regulate intranuclear mobility and distribution of Fos proteins.
- Suggests existence of intranuclear storage sites for latent c-Fos.c-Jun AP-1 complexes.
- This regulation impacts physiopathological functions controlled by these transcription factors.
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