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Updated: Jun 17, 2026

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

Chemokine binding proteins encoded by pathogens.

Antonio Alcami¹, Margarida Saraiva

¹Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones científicas, Universidad Autónoma de Madrid, Madrid, Spain. aalcami@cbm.uam.es

Advances in Experimental Medicine and Biology

|January 9, 2010

Summary

This summary is machine-generated.

Pathogens use chemokine binding proteins to evade immune responses. These proteins block chemokine activity, impacting cell migration and host defense mechanisms against infections.

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Identification of Functional Protein Regions Through Chimeric Protein Construction

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Published on: January 8, 2019

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Last Updated: Jun 17, 2026

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

Identification of Functional Protein Regions Through Chimeric Protein Construction

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

Area of Science:

Immunology
Virology
Parasitology

Background:

Chemokines are crucial chemoattractant cytokines in immune responses.
Pathogens frequently express chemokine inhibitors to counteract host defenses.
Poxviruses and herpesviruses secrete chemokine binding proteins that neutralize chemokine functions.

Purpose of the Study:

To investigate the role of pathogen-derived chemokine binding proteins in modulating immune responses.
To understand how these proteins interfere with chemokine-mediated cell migration.
To explore the therapeutic potential of targeting chemokine binding proteins in inflammatory diseases.

Main Methods:

Analysis of secreted proteins from poxviruses and herpesviruses.
Biochemical assays to determine the binding affinity of viral proteins to chemokines.
Identification and characterization of soluble chemokine binding proteins from parasites and ticks.

Main Results:

Pathogen-secreted chemokine binding proteins exhibit high affinity for chemokines, blocking their activity.
These proteins are structurally distinct from host chemokine receptors.
Soluble chemokine binding proteins are found in diverse organisms, including parasites and arthropods.

Conclusions:

Chemokine binding proteins are a conserved mechanism employed by various pathogens to subvert host immunity.
The specificity of these inhibitors highlights key chemokines involved in anti-pathogen defense.
Targeting chemokine binding proteins offers potential therapeutic strategies for inflammatory and infectious diseases.