Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

Nucleic acids

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An International ASXL3 Natural History Study: Deep Phenotypic Analyses Including Detailed Reports of a Milder Phenotype, Novel Associations, and Clinical Recommendations.

American journal of medical genetics. Part A·2025

Same author

Integrated microfluidic multiple electrode aggregometry for point-of-care platelet function analysis.

Lab on a chip·2024

Same author

Chasing hypokalemic paresis with diagnostic challenges: bilateral adrenal adenoma.

QJM : monthly journal of the Association of Physicians·2024

Same author

Azimuthal Correlations within Exclusive Dijets with Large Momentum Transfer in Photon-Lead Collisions.

Physical review letters·2023

Same author

Static <i>in vitro</i> digestion model adapted to the general older adult population: an INFOGEST international consensus.

Food & function·2023

Same author

Bayesian approaches to include real-world data in clinical studies.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2023

Search research articles

Related Experiment Video

Updated: Jun 17, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

Challenges and potential for RNA nanoparticles (RNPs).

D Davis¹, U Akhtar, B Keaster

¹Department of Biomedical Science, Missouri State University, Springfield, MO 65897, USA.

Journal of Biomedical Nanotechnology

|January 9, 2010

Summary

This research explores RNA-based nanoparticles (RNPs) for delivering therapeutic RNA, particularly splice-site switching oligomers (SSOs), to treat infectious diseases and cancer.

More Related Videos

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

Related Experiment Videos

Last Updated: Jun 17, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

Area of Science:

Biotechnology and Nanomedicine
Molecular Therapeutics

Background:

Oligonucleotide and gene vaccine delivery is crucial for infectious disease and cancer therapy.
Protamine-DNA-gold complexes offer a potential delivery platform.
Alternative splicing presents a new therapeutic target for RNA-based approaches.

Purpose of the Study:

To review strategies for binding, stabilizing, and delivering RNA, specifically splice-site switching oligomers (SSOs), using RNA-based nanoparticles (RNPs).
To explore the potential of RNPs as a chemotherapeutic agent delivery system.
To adapt DNA-gold nanoparticle strategies for RNA delivery.

Main Methods:

Review of existing literature on RNA-protein interactions and nanoparticle delivery systems.
Discussion of protamine as a potential enhancing material for RNA association with nanoparticles.
Analysis of challenges and opportunities in RNA stabilization and delivery.

Main Results:

Protamine-DNA strategies may be adaptable for RNA and other nanoparticles.
RNA-based nanoparticles (RNPs) can leverage alternative splicing for targeted therapy.
Key issues for SSO delivery via RNPs are identified.

Conclusions:

RNA-based nanoparticles (RNPs) show promise for targeted delivery of splice-site switching oligomers (SSOs).
This approach could unlock the potential of RNPs as novel chemotherapeutic agents.
Further research is needed to optimize binding, stabilization, and delivery of RNA therapeutics.