Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodal mass spectrometry imaging identifies cell-type-specific metabolic and lipidomic variation in the mammalian liver.

Developmental cell·2024
Same author

Successive High-Resolution (H<sub>2</sub>O)<sub></sub>-GCIB and C<sub>60</sub>-SIMS Imaging Integrates Multi-Omics in Different Cell Types in Breast Cancer Tissue.

Analytical chemistry·2021
Same author

Multiomics Imaging Using High-Energy Water Gas Cluster Ion Beam Secondary Ion Mass Spectrometry [(H<sub>2</sub>O)<sub></sub>-GCIB-SIMS] of Frozen-Hydrated Cells and Tissue.

Analytical chemistry·2021
Same author

Direct Mapping of Phospholipid Ferroptotic Death Signals in Cells and Tissues by Gas Cluster Ion Beam Secondary Ion Mass Spectrometry (GCIB-SIMS).

Angewandte Chemie (International ed. in English)·2021
Same author

Metabolomics and mass spectrometry imaging reveal channeled de novo purine synthesis in cells.

Science (New York, N.Y.)·2020
Same author

Enhanced Ion Yields Using High Energy Water Cluster Beams for Secondary Ion Mass Spectrometry Analysis and Imaging.

Analytical chemistry·2019
Same journal

Coadsorption of Atmospheric Surface-Active Organics at the Aqueous Interface: A Molecular Dynamics Study.

Annual review of physical chemistry·2026
Same journal

Control of Chemical Reactions in Radiofrequency Ion Traps.

Annual review of physical chemistry·2026
Same journal

Theories of Chiral-Induced Spin Selectivity: A Pedagogical Overview.

Annual review of physical chemistry·2026
Same journal

Quantum Computing Beyond Ground-State Electronic Structure: A Review of Progress Toward Quantum Chemistry Out of the Ground State.

Annual review of physical chemistry·2026
Same journal

First-Principles Simulations of Chemical Transformations in Nanoporous Materials and Industrial Catalysts.

Annual review of physical chemistry·2026
Same journal

Structure and Dynamics of Microhydrated Complexes Revealed with Rotational Spectroscopy.

Annual review of physical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2026

Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)
11:09

Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)

Published on: January 2, 2015

Biological cluster mass spectrometry.

Nicholas Winograd1, Barbara J Garrison

  • 1Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania 16802, USA. nxw@psu.edu

Annual Review of Physical Chemistry
|January 9, 2010
PubMed
Summary
This summary is machine-generated.

Cluster ion probes, especially C(60), enhance secondary ion mass spectrometry sensitivity and molecular analysis. This enables advanced 2D and 3D bioimaging of biological tissues and single cells.

More Related Videos

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
11:13

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products

Published on: March 12, 2020

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)
09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

Published on: October 30, 2016

Related Experiment Videos

Last Updated: Jun 17, 2026

Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)
11:09

Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)

Published on: January 2, 2015

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
11:13

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products

Published on: March 12, 2020

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)
09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

Published on: October 30, 2016

Area of Science:

  • Analytical Chemistry
  • Surface Science
  • Biophysics

Background:

  • Secondary ion mass spectrometry (SIMS) is a powerful surface analysis technique.
  • Traditional cluster ion probes offer some advantages but have limitations in molecular desorption and imaging.
  • Understanding energy deposition is key to improving SIMS performance.

Purpose of the Study:

  • To review the physics and applications of cluster ion probes in SIMS.
  • To highlight the enhanced molecular desorption and sensitivity achieved with cluster probes like C(60).
  • To explore the potential of microfocused cluster beams for 2D and 3D bioimaging.

Main Methods:

  • Utilizing cluster ion probes, particularly C(60), for enhanced molecular desorption in SIMS.
  • Applying microfocused cluster beams for high-resolution spatial analysis.
  • Developing and applying theoretical models to understand energy deposition.
  • Conducting experiments on model systems and biological samples.

Main Results:

  • Cluster ion probes, especially C(60), demonstrate improved sensitivity and molecular retention during desorption.
  • Microfocused beams enable feasible 2D and 3D bioimaging of molecular solids.
  • Emerging theoretical models provide atomic and molecular-level understanding of energy deposition.
  • Successful implementation of imaging protocols on biological tissue and single cells.

Conclusions:

  • Cluster ion probes represent a significant advancement in SIMS, offering enhanced sensitivity and molecular specificity.
  • The technology facilitates high-resolution 2D and 3D bioimaging, opening new avenues in biological and medical research.
  • Continued development of theoretical models and experimental protocols will further expand the applications of this methodology.