Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nanoporous silicon nitride membranes fabricated from porous nanocrystalline silicon templates.

Nanoscale·2014
Same author

Ballistic and non-ballistic gas flow through ultrathin nanopores.

Nanotechnology·2012
Same author

Role of glucosyltransferase B in interactions of Candida albicans with Streptococcus mutans and with an experimental pellicle on hydroxyapatite surfaces.

Applied and environmental microbiology·2011
Same author

Methods for controlling the pore properties of ultra-thin nanocrystalline silicon membranes.

Journal of physics. Condensed matter : an Institute of Physics journal·2011
Same author

An experimental and theoretical analysis of molecular separations by diffusion through ultrathin nanoporous membranes.

Journal of membrane science·2011
Same author

Lack of association of the WRN C1367T polymorphism with senile cataract in the Israeli population.

Molecular vision·2010
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
Same journal

Modular training resources for bioimage analysis.

Journal of microscopy·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Image correlation microscopy for uniform illumination.

T R Gaborski1, M N Sealander, M Ehrenberg

  • 1Department of Biomedical Engineering, School of Engineering Applied Science, University of Rochester, Rochester, NY 14627, USA.

Journal of Microscopy
|January 9, 2010
PubMed
Summary
This summary is machine-generated.

Uniform illumination image correlation microscopy extends fluorescence dynamics analysis to wide-field systems. This method offers greater temporal resolution and simplifies measurements of particle diffusion and aggregation.

More Related Videos

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
10:25

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Published on: September 27, 2024

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Related Experiment Videos

Last Updated: Jun 17, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
10:25

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Published on: September 27, 2024

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Area of Science:

  • Biophysics
  • Microscopy techniques
  • Cellular dynamics

Background:

  • Image cross-correlation microscopy traditionally uses laser-scanning systems, extending fluorescence correlation spectroscopy.
  • Laser-scanning microscopy limits temporal resolution and requires complex instrumentation.

Purpose of the Study:

  • To adapt image cross-correlation microscopy for uniform illumination/wide-field imaging systems.
  • To establish the quantitative relationships for uniform illumination image correlation microscopy.
  • To analyze cellular processes like adhesion molecule dynamics.

Main Methods:

  • Developed uniform illumination image correlation microscopy (UI-ICM).
  • Employed analytical, Monte Carlo, and experimental validations.
  • Utilized particle tracking algorithms for validation.
  • Applied UI-ICM to study neutrophil adhesion molecule aggregation and diffusion.

Main Results:

  • Demonstrated that UI-ICM accurately quantifies fluorescence dynamics in wide-field systems.
  • Established relationships between spatial/temporal autocorrelation functions, feature size, and diffusion coefficient.
  • Showed temporal autocorrelation function is dependent on particle size, not shape.
  • Validated UI-ICM with experimental data on human neutrophils.

Conclusions:

  • Uniform illumination image correlation microscopy is a viable, simpler, and faster alternative to laser-scanning methods.
  • UI-ICM enables precise measurement of diffusion coefficients and particle dynamics.
  • The technique is applicable to studying complex biological systems, such as membrane protein aggregation.