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Related Experiment Video

Updated: Jun 17, 2026

Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs

Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs

Published on: March 18, 2016

Silver impairs neurodevelopment: studies in PC12 cells.

Christina M Powers¹, Nicola Wrench, Ian T Ryde

¹Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.

Environmental Health Perspectives

|January 9, 2010

Summary

This summary is machine-generated.

Silver ions (Ag+) show potential for developmental neurotoxicity, acting more potently than the pesticide chlorpyrifos (CPF). Lower exposures reveal distinct effects, impacting cell differentiation and survival differently than outright toxicity.

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Last Updated: Jun 17, 2026

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Published on: March 18, 2016

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Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

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Published on: June 17, 2015

Area of Science:

Neuroscience
Toxicology
Cell Biology

Background:

Increasing exposure to silver nanoparticles in consumer products necessitates understanding silver's biological effects.
Monovalent silver ions (Ag+) are implicated in many biological effects of silver.

Purpose of the Study:

To evaluate silver's potential as a developmental neurotoxicant using neuronotypic PC12 cells.
To compare silver's neurotoxic effects with chlorpyrifos (CPF), a known developmental neurotoxicant.

Main Methods:

PC12 cells were used to assess the effects of silver ions (Ag+) and CPF on cell proliferation, protein synthesis, oxidative stress, and differentiation.
Dose-response relationships and effects on different cell states (undifferentiated vs. differentiating) were examined.

Main Results:

Silver ions (Ag+) inhibited DNA and protein synthesis more potently than CPF in undifferentiated cells.
In differentiating cells, Ag+ caused greater DNA synthesis inhibition, oxidative stress, impaired neurite formation, and altered neurotransmitter phenotype development.
Lower Ag+ concentrations (1 microM) differentially affected differentiating cells by suppressing cell death and impairing differentiation.

Conclusions:

Silver demonstrates potential for developmental neurotoxicity, exceeding the potency of CPF.
The effects of silver are concentration-dependent and context-specific, with lower exposures eliciting distinct outcomes that differ from overt toxicity.