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Updated: Jan 28, 2026

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
Published on: March 19, 2014
Purification and biochemical characterization of recombinant rice UDP-arabinopyranose mutase generated in insect
Tomoyuki Konishi1, Yasumasa Miyazaki, Seiyei Yamakawa
1Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan.
Abstract:
Plants utilize UDP-arabinofuranose (UDP-Araf) in the biosynthesis of Araf-containing complex carbohydrates. UDP-Araf is synthesized from UDP-arabinopyranose by UDP-arabinopyranose mutases (UAMs). Here we describe the heterologous expression of rice (Oryza sativa) UAM genes in insect cells and report some of their enzymatic properties. Recombinant UAMs might serve as useful tools for the biosynthesis of UDP-Araf and might be better than chemical synthesis.
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