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Updated: Jun 17, 2026

High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Culm in rice straw as a new source for sugar recovery via enzymatic saccharification
Jeung-yil Park1, Mitsuhiro Arakane, Riki Shiroma
1National Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan.
Abstract:
Rice straw was manually dissected and two main fractions were recovered: a culm and a leaf sheath/blade fraction, in order to evaluate their potential as feedstocks for the recovery of fermentable sugars. In the case of cv. Koshihikari and Milkyqueen, most soft carbohydrates (SCs: glucose, fructose, sucrose, starch, and beta-1,3-1,4-glucan) were present in the culms, reaching 47.9% and 89.2% of total SCs in the two main fractions. The results also indicated that beta-glucans (cellulose and beta-1,3-1,4-glucan) and xylan in the culms were more susceptible to direct enzymatic attack than those in the leaf sheath/blades. Thus the culm has high potential as a new feedstock for the extraction of fermentable sugars in a concentrated form, as compared to whole rice straw and the leaf sheath/blade. In this study, a novel method of separating a culm from the whole rice straw by means of wind power was also evaluated.
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