Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cognitive engagement induces area-specific fingerprints of dopamine, acetylcholine, serotonin, glutamate and GABA in prefrontal cortex and striatum.

bioRxiv : the preprint server for biology·2026
Same author

Nonexhaustive microextraction as a step toward more sustainable chemical analysis in the field and the clinic.

Nature protocols·2026
Same author

<i>In Vivo</i> Negligible Depletion SPME for the Determination of Free and Total Concentrations of Anandamide and 2-Arachidonoylglycerol in the Brain of a Parkinson's Disease Rat Model.

Analytical chemistry·2026
Same author

Defects in auxiliary fuel oxidation and mitochondrial pyruvate transport mark transition to overt heart failure in Tgαq*44 mice.

Journal of translational medicine·2026
Same author

High-throughput screening of per- and polyfluoroalkyl substances in human plasma using biocompatible solid-phase microextraction coupled with mass spectrometry via microfluidic open interface.

Analytica chimica acta·2026
Same author

Comprehensive analysis of exhaled breath VOCs using GC-MS and GC×GC-TOF-MS: a comparative platform evaluation with TFME and NTD sampling for free and total concentrations.

Analytical and bioanalytical chemistry·2025
Same journal

iMUT-seq mapping of DSB-induced mutations with high sensitivity at single-nucleotide resolution.

Nature protocols·2026
Same journal

An assay to quantify sexual commitment and stage conversion in the human malaria parasite Plasmodium falciparum.

Nature protocols·2026
Same journal

Author Correction: Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells.

Nature protocols·2026
Same journal

High-throughput measurements of protein domain functions using magnetic separation.

Nature protocols·2026
Same journal

Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.

Nature protocols·2026
Same journal

Photocatalytic low-temperature defluorination of PTFE.

Nature protocols·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2026

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
17:39

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

Published on: July 16, 2017

Protocol for solid-phase microextraction method development.

Sanja Risticevic1, Heather Lord, Tadeusz Górecki

  • 1Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada.

Nature Protocols
|January 9, 2010
PubMed
Summary
This summary is machine-generated.

Solid-phase microextraction (SPME) offers a solvent-free method for rapid sample preparation, integrating multiple analytical steps. This protocol details SPME optimization for developing high-quality analytical methods across various applications.

More Related Videos

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
07:26

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

Published on: January 1, 2016

The MPLEx Protocol for Multi-omic Analyses of Soil Samples
10:12

The MPLEx Protocol for Multi-omic Analyses of Soil Samples

Published on: May 30, 2018

Related Experiment Videos

Last Updated: Jun 17, 2026

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
17:39

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

Published on: July 16, 2017

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
07:26

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

Published on: January 1, 2016

The MPLEx Protocol for Multi-omic Analyses of Soil Samples
10:12

The MPLEx Protocol for Multi-omic Analyses of Soil Samples

Published on: May 30, 2018

Area of Science:

  • Analytical Chemistry
  • Sample Preparation Techniques

Background:

  • Solid-phase microextraction (SPME) is a versatile sample preparation technique.
  • It integrates sampling, extraction, concentration, and sample introduction into a single, solvent-free step.
  • SPME has been widely adopted due to its efficiency and compatibility with advanced analytical systems.

Purpose of the Study:

  • To provide a detailed protocol for optimizing SPME parameters.
  • To facilitate the development of high-quality analytical methods using SPME.
  • To address the need for systematic optimization in diverse analytical applications.

Main Methods:

  • Optimization of parameters affecting SPME extraction efficiency.
  • Development of a sequential protocol for method development.
  • Application of the protocol to various analytical challenges.

Main Results:

  • A comprehensive guide for SPME method optimization is presented.
  • The protocol enables enhanced extraction efficiency.
  • Facilitates the development of robust and reliable analytical methods.

Conclusions:

  • Optimized SPME protocols are crucial for high-quality analytical results.
  • This detailed sequence aids in developing effective SPME methods.
  • The protocol supports broad applicability in analytical chemistry.