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Updated: Jun 17, 2026

Linearization of the Bradford Protein Assay

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Published on: April 12, 2010

Accurate method for determining photometric linearity.

¹Michelson Laboratory, China Lake,California 93555, USA.

|January 9, 2010

Summary

This summary is machine-generated.

A new method using three polarizers improves photometric linearity measurements to better than 0.1%. This technique offers a more accurate way to check detector response and calibrate optical filters, overcoming limitations of traditional filter-based methods.

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Area of Science:

Optical Physics
Metrology
Instrumentation

Background:

Accurate photometric measurements are crucial for scientific research.
Detector linearity, the proportionality of output to light flux, is essential.
Traditional methods using absorption filters have limited accuracy (around 1%).

Purpose of the Study:

To develop a more accurate method for assessing photometric detector linearity.
To improve upon the accuracy limitations of existing linearity testing techniques.
To provide a reliable method for calibrating optical filters, especially those with high optical densities.

Main Methods:

Utilized a system of three high-quality polarizers in series.
Kept the axes of the outer two polarizers parallel.
Rotated the middle polarizer to systematically vary light intensity.

Main Results:

Achieved photometric linearity determination with an accuracy better than 0.1%.
Significantly reduced errors associated with traditional filter-based methods.
Demonstrated accurate determination of standard filter transmission values.

Conclusions:

The three-polarizer system provides a highly accurate method for photometric linearity assessment.
This technique is particularly advantageous for calibrating filters with large optical densities.
The method offers a substantial improvement over conventional techniques for optical metrology.