Jove
Visualize
Contact Us

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and validation of a UPLC-MS/MS method for real-time neuropharmacokinetic monitoring of iboga alkaloids in rat brain.

Journal of pharmaceutical and biomedical analysis·2026
Same author

Retraction Note: mGluR5 hypofunction is integral to glutamatergic dysregulation in schizophrenia.

Molecular psychiatry·2025
Same author

Oxa-Iboga alkaloids lack cardiac risk and disrupt opioid use in animal models.

Nature communications·2024
Same author

Pharmacology and Therapeutic Potential of Benzothiazole Analogues for Cocaine Use Disorder.

Journal of medicinal chemistry·2023
Same author

Chronic haloperidol administration downregulates select BDNF transcript and protein levels in the dorsolateral prefrontal cortex of rhesus monkeys.

Frontiers in psychiatry·2023
Same author

Three diketomorpholines from a Penicillium sp. (strain G1071).

Phytochemistry·2021
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 17, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Modeling substance abuse for applications in proteomics.

Scott E Hemby1, Nilesh Tannu

  • 1Department of Physiology & Pharmacology and Psychiatry, Wake Forest University School of Medicine, Winston-Salem, NC, USA. shemby@wfubmc.edu

Methods in Molecular Biology (Clifton, N.J.)
|January 9, 2010
PubMed
Summary
This summary is machine-generated.

This study details rodent intravenous self-administration methods to understand drug addiction. It links drug reinforcement behaviors to specific brain protein changes, aiding medication development for addictive disorders.

More Related Videos

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
17:12

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)

Published on: December 20, 2010

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

Related Experiment Videos

Last Updated: Jun 17, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
17:12

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)

Published on: December 20, 2010

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

Area of Science:

  • Neuroscience
  • Pharmacology
  • Biochemistry

Background:

  • Modeling human addictive behaviors in animals is crucial for understanding drug intake mechanisms.
  • Intravenous self-administration in rodents models drug reinforcement and associated biochemical changes.
  • Identifying targets for medication development is essential for treating addictive disorders.

Purpose of the Study:

  • To provide a detailed methodology for rodent intravenous self-administration.
  • To outline protein isolation and preparation from rodent brain regions for proteomic analysis.
  • To exemplify cocaine-induced alterations in ionotropic glutamate receptor subunits.

Main Methods:

  • Rodent intravenous self-administration.
  • Protein isolation and preparation from dissected brain regions.
  • Western blot analysis and high-throughput proteomic analysis.

Main Results:

  • Demonstration of a methodology linking drug self-administration to biochemical changes.
  • Examples of cocaine-induced alterations in ionotropic glutamate receptor subunits.
  • Identification of specific brain regions involved in drug reinforcement.

Conclusions:

  • The described methodology enables the study of biochemical underpinnings of addictive behaviors.
  • This approach facilitates the identification of potential therapeutic targets for addiction.
  • Understanding molecular changes associated with drug reinforcement is key to developing effective treatments.