Related Experiment Video
Updated: Jun 17, 2026

Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain
Published on: October 24, 2017
Protein aggregate characterization in models of neurodegenerative disease
Andrew T N Tebbenkamp1, David R Borchelt
1Department of Neuroscience, McKnight Brain Institute of the University of Florida, Gainesville, FL, USA.
Abstract:
A pathological hallmark of many neurodegenerative diseases is the presence of protein aggregates. Transgenic mice that recapitulate this pathology are a valuable resource to isolate these proteins for detailed study. One aspect of our research program is to characterize and quantify aggregates beta-amyloid (Abeta) peptides, superoxide dismutase 1 (SOD1), and huntingtin (htt) that comprise pathologic lesions found in Alzheimer's disease, familial amyotrophic lateral sclerosis, and Huntington's disease, respectively. In this chapter, we describe methods, based on sequential detergent extraction and ultracentrifugation, to isolate and analyze these protein aggregates. These methods have been applied to human tissues to some extent, but have been highly useful in studies involving transgenic mouse models of these diseases.
Related Concept Videos
Amyloid Fibrils
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining, normally used to...
Amyloid Fibrils
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining, normally used to...
Alzheimer Disease ll: Pathophysiology
Parkinson Disease ll: Pathophysiology

