Related Experiment Video
Updated: Jun 17, 2026

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Published on: April 5, 2019
Separation of the neuroproteome by ion exchange chromatography
Brian F Fuller1, Andrew K Ottens
1Department of Anatomy, Virginia Commonwealth University, Richmond, VA, USA.
Abstract:
Ion exchange chromatography is a fractionation technique applicable to the separation of brain-derived proteins based on charge. Proteome complexity overwhelms analytical approaches, which is mitigated by fractioning samples into simpler solutions. In this chapter we will cover the design, optimization, and execution of an ion exchange experiment for the separation of a brain lysate. Furthermore, helpful tips and pitfall will be revealed to aid with potential problems that may arise. The discussed proteomic methodology is applicable to multidimensional separations ahead of bottom-up or top-down proteomic strategies for characterizing the neuroproteome.
More Related Videos
10:51Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
Published on: April 10, 2014
09:57Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
Published on: June 14, 2020
Related Concept Videos
Ion-Exchange Chromatography
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Ion Exchange