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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: Jun 17, 2026

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth
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Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

Published on: November 10, 2023

A novel stem cell tag-less sorting method.

Barbara Roda1, Giacomo Lanzoni, Francesco Alviano

  • 1Department of Chemistry G. Ciamician, I-40126, Bologna, Italy.

Stem Cell Reviews and Reports
|January 9, 2010
PubMed
Summary

This study introduces a new, tag-less method for purifying mesenchymal stem cells (MSCs). This flow-assisted protocol simplifies cell isolation and characterization for improved cell therapy applications.

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Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth
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Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Area of Science:

  • Biotechnology
  • Cell Biology
  • Regenerative Medicine

Background:

  • Standardization in stem cell isolation is crucial for successful applications.
  • Mesenchymal stem cells (MSCs) are key candidates for cell therapy.
  • Current isolation methods often lack homogeneity and require extensive manipulation.

Purpose of the Study:

  • To present a novel tag-less, flow-assisted protocol for purifying, distinguishing, and sorting MSCs.
  • To simplify MSC isolation procedures, reduce manipulation, and enhance cell characterization.
  • To demonstrate the protocol's utility in various MSC applications.

Main Methods:

  • A suspension of cells in transport fluid is injected into a ribbon-like capillary device.
  • Continuous flow facilitates cell purification and sorting within approximately 30 minutes.
  • The method is tag-less, requiring minimal instrumental complexity.

Main Results:

  • The protocol effectively purifies MSCs from epithelial contaminants.
  • It enables characterization of MSCs from diverse human sources.
  • Subpopulations of MSCs with high differentiation potential were successfully sorted.

Conclusions:

  • The developed protocol offers a low-cost, biocompatible, and scalable approach for MSC selection.
  • This method enhances standardization and analytical information in stem cell research.
  • It holds significant potential for advancing cell therapy applications through improved MSC isolation.