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Reliability and reproducibility of macular segmentation using a custom-built optical coherence tomography retinal
Delia Cabrera DeBuc1, Gábor Márk Somfai, Sudarshan Ranganathan
1University of Miami, Miller School of Medicine, Bascom Palmer Eye Institute, 1638 North West 10th Avenue, Miami, Florida 33136, USA. dcabrera2@med.miami.edu
Journal of Biomedical Optics
|January 12, 2010
Summary
OCTRIMA software demonstrates high reliability for retinal thickness measurements. This optical coherence tomography analysis tool provides reproducible results for macular and intraretinal layers in healthy subjects.
Area of Science:
- Ophthalmology
- Medical Imaging
- Biomedical Engineering
Background:
- Accurate retinal thickness measurements are crucial for diagnosing and monitoring various eye diseases.
- Existing optical coherence tomography (OCT) analysis software may have limitations in reliability and reproducibility.
Purpose of the Study:
- To evaluate the reliability and reproducibility of a custom-built OCT retinal image analysis software (OCTRIMA).
- To assess the performance of OCTRIMA for quantifying macular and intraretinal layer thickness.
Main Methods:
- Ten eyes from five healthy subjects were scanned multiple times using a Stratus OCT device.
- OCTRIMA software was used for automatic/semi-automatic thickness quantification.
- Intraobserver, interobserver, and intervisit repeatability and reproducibility coefficients, along with intraclass correlation coefficients (ICCs), were calculated.
Main Results:
- Combined intraobserver, interobserver, and intervisit variability accounted for less than 5% of total variability in retinal thickness measurements.
- Variability for intraretinal layers was less than 7%, excluding the outer segment/retinal pigment epithelium (RPE) junction.
- High ICCs (greater than 0.75) were observed for most measurements, indicating strong agreement.
Conclusions:
- OCTRIMA software provides highly repeatable and reproducible retinal thickness measurements.
- The software is reliable for analyzing the total retina and most intraretinal layers.
- OCTRIMA shows potential as a valuable tool in ophthalmological research and clinical practice.
