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Updated: Jun 17, 2026

Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection
Published on: September 14, 2019
[Oocysts from Cryptosporidium spp. isolated from cattle: pleomorfism in relationship to diagnosis methodology]
Carina Dos S Teixeira1, Adriana J De Almeida, Francisco Carlos R De Oliveira
1Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, RJ 28013-600, Brazil.
Abstract:
The aim of this study was to analyze the pleomorfism of Cryptosporidium spp. oocysts isolated from calves feces throughout the comparison of two diagnostic techniques. One hundred of cattle feces were collected and the samples were submitted to both flotation sucrose centrifugation and to Ziehl-Neelsen modified tests. The results obtained showed a statistically significant reduction on the size of the Cryptosporidium oocysts observed through Ziehl-Neelsen technique when compared to the flotation sucrose centrifugation technique, and that the difference on the diameter of the oocysts suggests the there were different species of Cryptosporidium circulating in the animals analyzed.
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