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Related Experiment Video

Updated: Jun 17, 2026

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

Published on: June 2, 2019

A poly(dimethylsiloxane)-based device enabling time-lapse imaging with high spatial resolution.

Masahiko Hirano¹, Tetsushi Hoshida, Asako Sakaue-Sawano

¹Japan Science and Technology Agency, Wako, Saitama 351-0198, Japan. mhirano@postman.riken.jp

Biochemical and Biophysical Research Communications

|January 12, 2010

Summary

A new regulator-free device enables long-term mammalian cell incubation for epi-fluorescence imaging. This system maintains physiological conditions, allowing cells to proliferate normally for extended imaging periods.

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Last Updated: Jun 17, 2026

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Published on: December 25, 2015

Area of Science:

Cell Biology
Microscopy Techniques
Biomedical Engineering

Background:

Long-term cell culture for imaging requires stable physiological conditions.
Existing incubation systems can be bulky and complex, limiting their use with high-resolution microscopy.

Purpose of the Study:

To develop a compact, regulator-free device for long-term mammalian cell incubation suitable for epi-fluorescence imaging.
To maintain physiological pH and temperature for extended cell culture periods within a microscopy setup.

Main Methods:

A novel device utilizing a poly(dimethylsiloxane) (PDMS) block on a coverslip to supply CO(2)-rich gas.
A micro-chamber design to maintain bicarbonate-containing medium at physiological pH.
An oil-immersion objective lens used for both imaging and localized heating of the cells.

Main Results:

The device successfully enabled long-term incubation of mammalian cells (HeLa) for over 2 days.
Time-lapse imaging demonstrated normal cell proliferation and cell-cycle progression within the device.
The system maintained physiological pH and temperature for the duration of the experiment.

Conclusions:

The developed device offers a simple, regulator-free solution for long-term cell incubation and imaging.
This technology facilitates extended live-cell imaging studies by providing a stable microenvironment.
The findings support the use of this device for various applications in cell biology research.