Related Experiment Video

Updated: Jun 17, 2026

Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels
10:57

Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels

Published on: August 3, 2009

Direct red 81 and amido black stain proteins in polyacrylamide electrophoresis gels within 10 min

Ikechukwu Achilonu1, J P Dean Goldring

  • 1Biochemistry, University of KwaZulu-Natal, P.B. X01 Scottsville 3209, South Africa.

Analytical Biochemistry
|January 12, 2010
PubMed

Proteins separated by SDS-polyacrylamide gel electrophoresis can be stained with organic dyes, the most popular being Coomassie brilliant blue R-250. Coomassie R-250 staining of ovalbumin in an SDS-PAGE gel increased linearly from 2.5 to 60 min. Direct red 81 and amido black staining approached saturation in 10 min. Scatchard analysis showed that the number of direct red 81 and amido black ligands bound to ovalbumin was fourfold higher than that of Coomassie R-250. Direct red 81 and amido black stain proteins in an SDS-polyacrylamide electrophoresis gel in 10 min.

More Related Videos

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels06:24

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

Staining Proteins in Gels10:55

Staining Proteins in Gels

Published on: July 8, 2008

Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...