Butin reduces oxidative stress-induced mitochondrial dysfunction via scavenging of reactive oxygen species

This study investigated the cytoprotective effect of butin, a flavonoid, on hydrogen peroxide (H(2)O(2))-induced mitochondrial dysfunction. Electron spin resonance (ESR) spectrometry revealed butin's significant scavenging effects on superoxide radicals and hydroxyl radicals. When H(2)O(2) was used to induce an increase in mitochondrial reactive oxygen species (ROS) in Chinese hamster lung fibroblast (V79-4) cells, butin treatment decreased high level of ROS. Butin also attenuated intracellular Ca(2+) levels that have been induced by H(2)O(2). Furthermore, butin recovered ATP levels and succinate dehydrogenase activity that had been decreased by H(2)O(2) treatment. We conclude these results suggest butin decreased mitochondrial ROS accumulation, balanced intracellular Ca(2+) levels, and improved mitochondrial energy production, thus recovering mitochondrial function.