Related Experiment Video
Updated: Jun 17, 2026

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
Ligninolytic and antioxidative enzymes of a marine cyanobacterium Oscillatoria willei BDU 130511 during Poly R-478
Sushanta Kumar Saha1, Palanisami Swaminathan, C Raghavan
1National Facility for Marine Cyanobacteria, Bharathidasan University, Tiruchirappalli 620024, India. susaha@ucsd.edu
Abstract:
Removal of combined nitrogen and addition of Poly R-478 to the growth medium enhanced oxidative stress, and altered the activities of ligninolytic enzymes of Oscillatoria willei BDU 130511. The activities of ligninolytic and antioxidative enzymes (LiP-like, LAC, PPO, SOD, POD, CAT, and APX) were increased upon nitrogen limitation and dye supplementation. The metabolic enzymes tested (GR, GPX, EST, and MDH) showed differential expressions under varied growth conditions. Up on nitrogen limitation, O. willei BDU 130511 showed enhanced ligninolytic activity as shown by alpha-keto-gamma-methylthiolbutyric acid (KTBA) oxidation and increased H(2)O(2) production. The organism decolourized 52% of Poly R-478 due to partial degradation and adsorption of dye particles from dye-added medium after 7 days of growth. This manuscript discusses the responses of ligninolytic and antioxidative enzymes of O. willei BDU 130511 during Poly R-478 decolourization/degradation, and the organism's potential in bioremediation.
More Related Videos
Related Concept Videos
Bioremediation
Microbial Bioremediation of Hydrocarbons
Bacterial Phylum Cyanobacteria
Microbial Bioremediation of Pesticides

