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Related Experiment Videos

An angiogenin-binding protein from endothelial cells.

G F Hu1, S I Chang, J F Riordan

  • 1Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, MA 02115.

Proceedings of the National Academy of Sciences of the United States of America
|March 15, 1991
PubMed
Summary

Researchers identified a novel angiogenin binding protein (AngBP) on endothelial cells. This protein may function as a receptor involved in angiogenesis, the formation of new blood vessels.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Endothelial cells play a crucial role in angiogenesis.
  • Angiogenin is a key protein involved in blood vessel formation.
  • The specific mechanisms of angiogenin's action at the cellular level are not fully understood.

Purpose of the Study:

  • To identify and characterize proteins that bind to bovine angiogenin on endothelial cells.
  • To investigate the potential role of angiogenin binding proteins in angiogenesis.

Main Methods:

  • Isolation and characterization of a 42-kDa bovine protein (AngBP) from endothelial cells.
  • Binding assays using radiolabeled bovine angiogenin and AngBP.
  • Competition assays with unlabeled angiogenin, ribonuclease A, and lysozyme.

Related Experiment Videos

  • Crosslinking studies using a carbodiimide.
  • Affinity chromatography and HPLC for AngBP purification.

    • Main Results:

    • A 42-kDa AngBP was identified on calf pulmonary artery endothelial cells and a transformed cell line.
    • AngBP specifically binds bovine angiogenin with high affinity (apparent Kd ~5 x 10(-10) M).
    • Binding is saturable and inhibited by excess unlabeled angiogenin.
    • Enzymatically active angiogenin derivatives that do not induce angiogenesis bind weakly to AngBP.
    • AngBP exhibits characteristics of a receptor.

    Conclusions:

    • A novel angiogenin binding protein (AngBP) has been identified on endothelial cells.
    • AngBP likely functions as a receptor for angiogenin.
    • This interaction may play a significant role in the process of angiogenesis.