Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methylation patterns of testis-specific genes.

M Ariel1, J McCarrey, H Cedar

  • 1Department of Cellular Biochemistry, Hebrew University Medical School, Jerusalem, Israel.

Proceedings of the National Academy of Sciences of the United States of America
|March 15, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of the trochlear nerve in eye abduction and frontal vision of the red-eared slider turtle (Trachemys scripta elegans).

The Journal of comparative neurology·2013
Same author

Gene repression paradigms in animal cells.

Cold Spring Harbor symposia on quantitative biology·2005
Same author

Quantitative analysis of granule cell axons and climbing fiber afferents in the turtle cerebellar cortex.

Anatomy and embryology·2004
Same author

A PCR-based method for studying DNA methylation.

Methods in molecular biology (Clifton, N.J.)·2003
Same author

Epigenetic crosstalk.

Molecular cell·2001
Same author

Direction tuning of inhibitory inputs to the turtle accessory optic system.

Journal of neurophysiology·2001
Same journal

The TaMYB55-TaSnRK1α1-TabZIP9 module confers heat stress tolerance in wheat.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Superstatistics approach to turbulent circulation fluctuations.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

A molecular timescale for evolution of cobamide biosynthesis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Pierre Chambon, a pioneer of molecular biology and gene regulation in eukaryotes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Granulosa cell glycogen fuels the avascular corpus luteum.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Synthetic essentiality of TRAIL/TNFSF10 in VHL-deficient renal cell carcinoma.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Mouse male germ line gene methylation patterns were studied. Sperm DNA methylation patterns are similar to somatic tissues, with no germ-line-specific unmethylated sites.

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Gene methylation patterns are crucial for regulating gene expression.
  • Understanding DNA methylation in the male germ line is essential for reproductive health and development.
  • Specific genes exhibit distinct methylation profiles during spermatogenesis.

Purpose of the Study:

  • To investigate DNA methylation patterns in mouse male germ line-specific genes.
  • To compare methylation status in somatic tissues, germ cells, and mature sperm.
  • To identify potential regulatory roles of methylation in spermatogenesis.

Main Methods:

  • Analysis of DNA methylation using techniques like bisulfite sequencing.
  • Examination of gene expression and methylation across different stages of spermatogenesis.

Related Experiment Videos

  • Comparison of methylation patterns in somatic cells versus germ line cells.

    • Main Results:

    • Testis-specific genes with 5' CpG islands (e.g., Int-1, Hox-2.1, Prm-1) are largely unmethylated in somatic tissues and sperm.
    • The non-CpG island gene Pgk-2 is methylated in non-expressing cells but undermethylated during active transcription in spermatocytes and spermatids.
    • Pgk-2 becomes remethylated in later spermatogenesis stages, resulting in a somatic-like methylation pattern in sperm DNA.

    Conclusions:

    • Sperm DNA methylation patterns are generally similar to somatic tissues, lacking germ-line-specific unmethylated sites.
    • Constitutively unmethylated genes in the germ line may possess specific regulatory functions.
    • Dynamic methylation changes during spermatogenesis ensure the final sperm epigenome is comparable to somatic cells.