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Updated: Jun 17, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
Cdk5, the multifunctional surveyor
Vassiliki Lalioti1, Diego Pulido, Ignacio V Sandoval
1Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Centro de Enfermedades Hepáticas y Digestivas, Instituto de Salud Carlos III, Madrid, Spain.
Abstract:
Cyclin-dependent kinases (Cdks) are a family of proline-directed Ser/Thr kinases known for their role in the control of cell cycle progression. In 1992, this family was joined by Cdk5, which is an atypical member in that it uses its own activators and is multifunctional, playing important regulatory roles in multiple cellular functions. Here, we review the structure of Cdk5 and its various mechanisms of activation within the context of the Cdk family. We also address the functional significance of the numerous and varied targets of Cdk5 within pathways that control the organization of the cytoskeleton and focal adhesions, signaling cascades, membrane dynamics and function, cell metabolism, cycle arrest in postmitotic cells, gene transcription and cell survival, all within the context of the adaptation of cells and life systems to changing internal and external environments.
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