Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Upsampling01:22

Upsampling

Managing signal sampling rates is essential in digital signal processing to maintain signal integrity. A decimated signal, characterized by a reduced frequency range due to its lower sampling rate, can be upsampled by inserting zeros between each sample. This upsampling process expands the original spectrum and introduces repeated spectral replicas at intervals dictated by the new Nyquist frequency. To refine this zero-inserted sequence, it is passed through a lowpass filter with a cutoff...
Aliasing01:18

Aliasing

Accurate signal sampling and reconstruction are crucial in various signal-processing applications. A time-domain signal's spectrum can be revealed using its Fourier transform. When this signal is sampled at a specific frequency, it results in multiple scaled replicas of the original spectrum in the frequency domain. The spacing of these replicas is determined by the sampling frequency.
If the sampling frequency is below the Nyquist rate, these replicas overlap, preventing the original signal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Null curves in diffraction patterns.

Applied optics·2010
Same author

Optical applications of von Neumann's alternating-projection theorem.

Optics letters·2009
Same author

Restoration of images possessing a finite Fourier series.

Optics letters·2009
Same author

Restoration of images possessing a finite Fourier seriesa: errata.

Optics letters·2009
Same author

Unitary operators in the homogeneous wave field.

Optics letters·2009
Same author

Phase retrieval and the polarization identity.

Optics letters·2009
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Increased optical resolution from a sampled wavefront.

W D Montgomery1

  • 1Institute for Defense Analyses, Arlington,Virginia 22202, USA.

Applied Optics
|January 12, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a novel holographic method for capturing scattered light wavefronts using short laser pulses and internal reference holograms. Polarization filtering effectively removes unwanted specular reflections during reconstruction.

More Related Videos

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Related Experiment Videos

Last Updated: Jun 17, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Area of Science:

  • Optics and Photonics
  • Holography
  • Laser Technology

Background:

  • Traditional holographic techniques often require long coherence length lasers.
  • Obtaining detailed wavefront information from scattered light can be challenging.
  • Specular reflections can interfere with holographic reconstructions.

Purpose of the Study:

  • To propose a holographic arrangement for capturing scattered wavefront samples.
  • To enable the use of short laser pulses with limited coherence length.
  • To develop a method for eliminating specular reflections in holographic reconstruction.

Main Methods:

  • Utilizing a holographic setup to capture scattered light wavefronts from a laser-illuminated object.
  • Generating an internal reference wave from specular reflections for each hologram sample.
  • Employing polarization filtering during the reconstruction phase.

Main Results:

  • Successful acquisition of wavefront samples using short laser pulses (coherence length of a few meters).
  • Demonstration of hologram formation using internally generated reference waves.
  • Effective suppression of specular reflections through polarization filtering in reconstruction.

Conclusions:

  • The proposed holographic arrangement is effective for capturing scattered wavefronts with short laser pulses.
  • Internal reference generation simplifies the holographic process.
  • Polarization filtering is a viable technique for removing artifacts in reconstruction.