Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
Gain01:15

Gain

Gain and phase shift are properties of linear circuits that describe the effect a circuit has on a sinusoidal input voltage or current. The circuit's behavior that contains reactive elements will depend on the frequency of the input sinusoid. As a result, it is observed that the gain and phase shift will all be frequency functions.
Gain:
Suppose Vin is the input and Vout is the output signal to a circuit.
Receiver Operating Characteristic Plot01:15

Receiver Operating Characteristic Plot

A ROC (Receiver Operating Characteristic) plot is a graphical tool used to assess the performance of a binary classification model by illustrating the trade-off between sensitivity (true positive rate) and specificity (false positive rate). By plotting sensitivity against 1 - specificity across various threshold settings, the ROC curve shows how well the model distinguishes between classes, with a curve closer to the top-left corner indicating a more accurate model. The area under the ROC curve...
Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolome remodeling with reverse-engineered exclusive enteral nutrition in children with active Crohn's disease.

Inflammatory bowel diseases·2026
Same author

Metabolomic signatures of dietary carbohydrates and differential association with type 2 diabetes.

Nature health·2026
Same author

Plant-based whole-food diets are feasible during auto-HCT and are associated with dose-dependent microbiome modulation.

Blood advances·2026
Same author

ssNetShift: single-sample metabolic network rewiring reveals hidden prognostic subtypes beyond clinical staging in gastric cancer.

Briefings in bioinformatics·2026
Same author

Variable Selection with FDR Control for Noisy Data - An Application to Screening Metabolites that Are Associated with Breast Cancer and Colorectal Cancer.

Journal of data science : JDS·2026
Same author

Correction: What's in a name? Metabolite identification: challenges and pitfalls in untargeted metabolomics.

Metabolomics : Official journal of the Metabolomic Society·2026

Related Experiment Video

Updated: Jun 17, 2026

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
09:25

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

Receiver gain function: the actual NMR receiver gain.

Huaping Mo1, John S Harwood, Daniel Raftery

  • 1Purdue Interdepartmental NMR Facility, Purdue University, West Lafayette, IN 47907, USA. hmo@purdue.edu

Magnetic Resonance in Chemistry : MRC
|January 12, 2010
PubMed
Summary
This summary is machine-generated.

Nuclear Magnetic Resonance (NMR) signal intensity is affected by receiver gain. We developed a receiver gain function to accurately quantify NMR signal amplification, enabling precise quantitative analysis across different gain settings.

More Related Videos

Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the Rescue
07:40

Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the Rescue

Published on: May 17, 2024

MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T
10:22

MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T

Published on: January 16, 2021

Related Experiment Videos

Last Updated: Jun 17, 2026

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
09:25

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the Rescue
07:40

Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the Rescue

Published on: May 17, 2024

MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T
10:22

MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T

Published on: January 16, 2021

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Nuclear Magnetic Resonance (NMR)

Background:

  • NMR signal intensity is influenced by the receiver gain setting.
  • Accurate quantitative analysis in NMR often necessitates varying receiver gain to prevent saturation or optimize sensitivity.
  • Discrepancies can exist between the suggested receiver gain setting and the actual receiver gain.

Purpose of the Study:

  • To introduce a receiver gain function for characterizing NMR signal amplification.
  • To enable accurate comparison of NMR signals acquired with different receiver gain settings.
  • To facilitate concentration determination using internal or external references.

Main Methods:

  • Development of a receiver gain function to model signal amplification.
  • Calibration of the receiver gain function for specific NMR instruments.
  • Integration of the receiver gain function with the concept of receiving efficiency.

Main Results:

  • Demonstration that the receiver gain function can be calibrated for a given receiver.
  • The calibrated function accurately describes signal amplification as a function of receiver gain.
  • The proposed method allows for precise quantitative analysis and concentration determination.

Conclusions:

  • The receiver gain function provides a method for correcting NMR signal intensities acquired at different gain settings.
  • Accurate NMR signal quantification is achievable through receiver gain calibration.
  • This approach simplifies concentration determination in quantitative NMR spectroscopy.