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Leishmaniasis

Leishmaniasis

Leishmaniasis is a protozoal disease caused by species of the genus Leishmania and transmitted through the bite of infected female sandflies. The parasite exists in two principal morphological forms during its life cycle. A sandfly acquires intracellular amastigotes from an infected reservoir host, such as a dog. Within the sandfly, these forms differentiate into motile, flagellated promastigotes. During a subsequent blood meal, promastigotes are injected into the human host, where they...

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Related Experiment Video

Updated: Jun 17, 2026

Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites

Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites

Published on: May 18, 2016

Systemic loxoscelism confirmation by bite-site skin surface: ELISA.

William V Stoecker¹, Gary S Wasserman, David A Calcara

¹Division of Dermatology, University of Missouri School of Medicine, MO, USA. wvs@mst.edu

Missouri Medicine

|January 13, 2010

Summary

This summary is machine-generated.

Systemic loxoscelism, a severe spider bite reaction, was confirmed using a novel skin surface ELISA test. This diagnostic method aids in identifying venom in cases where the spider is not found.

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Area of Science:

Toxicology
Clinical Medicine
Immunology

Background:

Loxoscelism, a condition caused by the venom of recluse spiders (Loxosceles spp.), can manifest with severe systemic effects.
Diagnosis of loxoscelism can be challenging, especially when the spider is not available for identification.

Observation:

A case of systemic loxoscelism presented with debilitating symptoms, a characteristic local bite reaction, and a symptomatic exanthem.
The patient experienced significant hemolysis, losing approximately 15% of their blood volume within 72 hours.

Findings:

A skin surface ELISA test was successfully employed to detect Loxosceles venom directly from the bite site.
This diagnostic approach confirmed the presence of systemic loxoscelism even without direct visualization of the spider.

Implications:

The skin surface ELISA test offers a valuable tool for the definitive diagnosis of loxoscelism.
This method can improve patient management and epidemiological understanding of recluse spider envenomation.