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Updated: Jun 17, 2026
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Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
Validation of a modified cryopreservation method for leukemic blasts for flow cytometry assessment
Huda Alsayed1, Tarek Owaidah, Faisal Al Rawas
1Department of Diagnostic Hematology, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Hematology/Oncology and Stem Cell Therapy
|January 13, 2010
Summary
Cryopreservation of human acute leukemia cells using 5% dimethysulfoxide is effective, maintaining high cell viability and antigenic expression for at least six weeks. This simple method supports retrospective studies of leukemia cell populations.
Area of Science:
- Hematology
- Cell Biology
- Immunology
Background:
- Cryopreservation is vital for retrospective studies of human cell populations.
- Frozen lymphocytes are valuable for immunological assays, tissue typing, and monitoring cell-mediated immunity.
- An efficient, simple, and cost-effective cryopreservation method for human acute leukemia cells is presented.
Purpose of the Study:
- To describe an efficient, simple, and inexpensive method for cryopreservation of human acute leukemia cells.
- To assess the viability and antigenic expression of cryopreserved leukemia cells over time.
Main Methods:
- Leukemia cells from 20 newly diagnosed cases were cryopreserved using 5% dimethysulfoxide at -80 degrees C.
- Antigen expression was analyzed by flow cytometry using monoclonal antibodies at various time points.
- Cell viability was assessed to determine the efficacy of the cryopreservation method.
Main Results:
- Initial viability across all leukemia subtypes (pre-B ALL, T-ALL, AML, biphenotypic) was above 75%.
- After 4 weeks, viability remained above 75% for 91% of pre-B ALL, 88% of T-ALL, and 100% of AML and biphenotypic samples.
- Cell viability consistently stayed above 75% for at least 6 weeks post-cryopreservation.
Conclusions:
- The described cryopreservation method is effective in maintaining high cell viability.
- The method preserves the antigenic expression of leukemia cells, crucial for immunological studies.
- This technique offers a reliable approach for storing leukemia cells for future research and clinical applications.
