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[Effect of Paenibacillus polymyxa lectin on cytokine status of animals]
Aim:
To study effect of lectin L II of Paenibacillus polymyxa 1460 on cytokine status of mice during modeling of infections caused by Staphylococcus, Escherichia and Yersinia.
Materials And Methods:
Lectin LII of P. polymyxa 1460, bacterial cultures Staphylococcus aureus 209-P, Escherichia coli O1, and Yersinia enterocolitica 12 were used. Mice were inoculated intraperitoneally with 0.2 ml of suspension of bacterial culture grown during 24 hours (5,000 m.c./ml). Lectin (0.4 mcg/ml) in dose 0.2 ml was administered 24 h before and 1 h after inoculation. Serum samplesfrom sacrificed animals were obtained 1, 6, and 24 hours after inoculation and concentrations of IL-1, IL-6, and TNF-alpha were measured in them using optical density values.
Results:
It was established that level of TNF-alpha in serum decreased during staphylococcal infection, whereas levels of IL-1 and IL-6 decreased during all modeled infections. Ability for correction of cytokine balance in organism of experimental animals by administration of lectin 24 h before and 1 h after inoculation with bacteria was demonstrated.
Conclusion:
Presented studies testify to the effect of bacterial lectin on cytokine-production activity of macrophages during phagocytosis of bacteria and infectious process.
Insights
Bacterial lectin L II from Paenibacillus polymyxa modulated mouse cytokine levels during Staphylococcus, Escherichia, and Yersinia infections. This lectin demonstrated potential in correcting cytokine balance disrupted by these pathogens.
Area of Science:
- Immunology
- Microbiology
- Biochemistry
Background:
- Cytokine profiles are critical in host defense against bacterial infections.
- Bacterial lectins can interact with host immune systems.
- Paenibacillus polymyxa lectin L II's immunomodulatory effects require investigation.
Purpose of the Study:
- To investigate the impact of Paenibacillus polymyxa 1460 lectin L II on mouse cytokine status.
- To assess the lectin's effect during modeled infections caused by Staphylococcus aureus, Escherichia coli, and Yersinia enterocolitica.
Main Methods:
- Mice were inoculated with Staphylococcus aureus, Escherichia coli, or Yersinia enterocolitica.
- Lectin L II was administered 24 hours before and 1 hour after bacterial inoculation.
- Serum concentrations of Interleukin-1 (IL-1), Interleukin-6 (IL-6), and Tumor Necrosis Factor-alpha (TNF-alpha) were measured.
Main Results:
- Tumor Necrosis Factor-alpha (TNF-alpha) levels decreased during Staphylococcus aureus infection.
- Interleukin-1 (IL-1) and Interleukin-6 (IL-6) levels decreased across all modeled infections.
- Lectin L II administration showed a corrective effect on the cytokine balance.
Conclusions:
- Bacterial lectin L II influences the cytokine production activity of macrophages.
- The lectin plays a role in modulating the immune response during bacterial phagocytosis and infection.
