Related Experiment Video
Updated: Jun 17, 2026
Protocol for Developing a Femur Osteotomy Model in Wistar Albino Rats
Published on: August 31, 2022
Development of a high-specificity enzyme-linked immunosorbent assay (ELISA) system for the quantification and
Hiroyuki Funaoka1, Yoshiko Dohi, Hajime Ohgushi
1Department of Public Health, Health Management and Policy, Nara Medical University School of Medicine, 840 Shijo-cho, Kashihara, Nara, 634-8521, Japan. hiroyuki-funaoka@bio.ds-pharma.co.jp
Abstract:
Osteocalcin (OC) exhibits hard tissue-specific expression and binding activity to hydroxyapatite. Therefore, measurement of secreted OC is a very useful index for evaluating osteoblastic differentiation in regenerative bone. In the present study, we established a high-specificity sandwich enzyme-linked immunosorbent assay (ELISA) system for the quantification of intact rat OC, which could be useful for validating tissue-engineered bone samples nondestructively and continuously. The range of detection with the sandwich ELISA system was 0.1-100 ng OC/mL of cell culture media or rat sera. No cross-reactivities were detected with OCs from other species, including human, bovine and mouse OCs, and other mammalian sera, which would contain the corresponding endogenous OCs. The intra- and inter-assay coefficients of variation were < or =4.9% and
