Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CaMKIIβ insufficiency disrupts cortical networks, producing aberrant low-gamma oscillations and seizure susceptibility.

Epilepsia·2026
Same author

Psychedelic 5-HT2A agonist increases spontaneous and evoked 5-Hz oscillations in visual and retrosplenial cortex.

Communications biology·2026
Same author

Cellular microenvironment of erythropoietin-producing cells in hypoxic and injured mouse kidneys.

Experimental physiology·2026
Same author

Lack of challenge to substantive criteria at mental health tribunals: Amplifying the medical perspective?

International journal of law and psychiatry·2025
Same author

Visibility and Invisibility of Nurses in Hospital Settings: An Analysis Based on the Sociologies of Ignorance and of Absences.

Nursing inquiry·2025
Same author

Inherent single-cell heterogeneity of the transcriptional response to hypoxia in cancer cells.

NAR cancer·2025
Same journal

The Hedgehog Pathway Effector Smoothened Exhibits Signaling Competency in the Absence of Ciliary Accumulation.

Chemistry & biology·2017
Same journal

DIVERSE System: De Novo Creation of Peptide Tags for Non-enzymatic Covalent Labeling by In Vitro Evolution for Protein Imaging Inside Living Cells.

Chemistry & biology·2015
Same journal

Differential Regulation of Specific Sphingolipids in Colon Cancer Cells during Staurosporine-Induced Apoptosis.

Chemistry & biology·2015
Same journal

Synthetic Peptides as cGMP-Independent Activators of cGMP-Dependent Protein Kinase Iα.

Chemistry & biology·2015
Same journal

Unraveling the B. pseudomallei Heptokinase WcbL: From Structure to Drug Discovery.

Chemistry & biology·2015
Same journal

Vitamin C as Cancer Destroyer, Investigating Sulfhydration, and the Variability in CFTR Interactome.

Chemistry & biology·2015
See all related articles

Related Experiment Video

Updated: Jun 17, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
11:51

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

Red-shifted voltage-sensitive fluorescent proteins.

Amelie Perron1, Hiroki Mutoh, Thomas Launey

  • 1Laboratory for Neuronal Circuit Dynamics, RIKEN Brain Science Institute, Wako-shi, Saitama 351-0198, Japan.

Chemistry & Biology
|January 13, 2010
PubMed
Summary
This summary is machine-generated.

Researchers developed new voltage-sensitive fluorescent proteins for imaging neural activity. These genetically encoded indicators allow optical recording of electrical signals in large neuronal populations, advancing brain circuit research.

More Related Videos

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes
06:59

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

Published on: September 27, 2018

Related Experiment Videos

Last Updated: Jun 17, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
11:51

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes
06:59

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

Published on: September 27, 2018

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biophysics

Background:

  • Electrical signals from neurons are fundamental to brain function.
  • Current methods like microelectrode recording are limited for studying large, heterogeneous neuronal circuits.
  • Optogenetic tools with improved spectral properties are needed for in vivo imaging of neuronal populations.

Purpose of the Study:

  • To develop novel genetically encoded voltage-sensitive fluorescent proteins (VSDs) for optical recording of neuronal electrical activity.
  • To optimize spectral properties for in vivo imaging applications.
  • To enable the study of neuronal circuit mechanisms involving large, heterogeneous neuron populations.

Main Methods:

  • Molecular fusion of the voltage-sensing domain of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP) to red-shifted fluorescent proteins.
  • Characterization of the generated genetically encoded voltage indicators.
  • Application in optical recording of electrical signals in cultured hippocampal neurons.

Main Results:

  • Successfully generated and characterized novel voltage-sensitive fluorescent proteins.
  • Demonstrated that these proteins convert voltage-dependent structural changes into fluorescence modulation.
  • Achieved optical recording of individual and simultaneous electrical signals in cultured neurons with single-cell resolution and without temporal averaging.

Conclusions:

  • The developed genetically encoded voltage indicators are effective tools for optical electrophysiology.
  • These indicators facilitate the study of neuronal circuit mechanisms at a larger scale than previously possible.
  • The findings offer a promising approach for advancing in vivo neuroscience research.