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Robust Detection of Gene Amplification in Formalin-Fixed Paraffin-Embedded Samples by Fluorescence In Situ Hybridization
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Fluorescent in situ hybridization as a primary test for HER2 status in breast cancer: controversies

Vincenzo Arena, Ilaria Pennacchia, Giovanni Monego

    Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology
    |January 13, 2010
    PubMed
    Abstract

    No abstract available in PubMed .

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    FISH - Fluorescent In-situ Hybridization02:07

    FISH - Fluorescent In-situ Hybridization

    Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
    In-situ Hybridization02:31

    In-situ Hybridization

    In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
    Types of probes and labels
    A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

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