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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Analyzers: Common Types01:19

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The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
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Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...

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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Published on: July 12, 2013

Imaging mass spectrometry using a delay-line detector.

Martin Froesch1, Stefan L Luxembourg, Duncan Verheijde

  • 1FOM Institute for Atomic and Molecular Physics, Science Park 104, 1098 XG Amsterdam, The Netherlands.

European Journal of Mass Spectrometry (Chichester, England)
|January 13, 2010
PubMed
Summary
This summary is machine-generated.

A novel delay-line detector enhances microscope mode mass spectrometry imaging. This advancement provides high-resolution, position-resolved detection for improved spatial analysis in MALDI-ToF and SIMS-ToF instruments.

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Area of Science:

  • Analytical Chemistry
  • Biophysics
  • Mass Spectrometry Imaging

Background:

  • Microscope mode mass spectrometric imaging requires high-resolution, position-resolved time-of-flight detectors.
  • Existing detectors may limit the spatial resolution and analytical capabilities of imaging mass spectrometry.

Purpose of the Study:

  • To introduce a new detection method for microscope mode imaging mass spectrometry.
  • To evaluate the performance of a delay-line detector as a position-sensitive detector in this application.

Main Methods:

  • Implementation of a delay-line detector in matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) and secondary ion mass spectrometry time-of-flight (SIMS-ToF) instruments.
  • Spatial resolution assessment using trypsinogen and bovine serum albumin samples with a metal mask on a MALDI-ToF instrument.
  • Generation of mass-resolved SIMS images from biological samples, including xenografted breast cancer cell line tumors and chicken embryonal sections.

Main Results:

  • The delay-line detector was successfully implemented as a position-sensitive detector in both MALDI-ToF and SIMS-ToF systems.
  • The spatial resolution of the detector was determined using standard protein samples.
  • Mass-resolved SIMS images of biological structures from tissue sections were successfully generated.

Conclusions:

  • The delay-line detector represents a significant advancement for microscope mode imaging mass spectrometry.
  • This new method enables high-resolution, position-resolved detection, enhancing the capabilities of MALDI-ToF and SIMS-ToF instruments.
  • The successful imaging of biological structures demonstrates the potential of this technique for analyzing complex biological samples.