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Updated: Jun 17, 2026

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
Published on: February 5, 2021
[Cloning and characterization of open reading frame encoding beta-amyrin synthase in Glycyrrhiza uralensis]
Zhanyun Shen1, Chunsheng Liu, Xueyong Wang
1School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Objective:
To clone and sequence the open reading frame of beta-amyrin synthase (bAS) from Glycyrrhiza uralensis.
Method:
The primers were designed according to the cDNA sequence of beta-amyrin synthase from G. glabra reported by Hiroaki HAYASHI, and the open reading frame of beta-amyrin synthase was cloned by RT-PCR strategy with the template of total RNA extracted from roots of G. uralensis.
Result:
The GubAS (GenBank Accession number: FJ627179) was 2 289 bp in length encoding one pelypeptide of 762 amino acid. Deduced amino acid sequence had 99%, 92%, 90%, 90% and 89% homology to the amino acid sequence of G. glabra, Lotus japonicus, Pisum sativum, Medicago truncatula, Glycine max, respectively.
Conclusion:
The open reading frame of bAS from G. uralensis is cloned and reported for the first time. The conclusion will provide a foundation for exploring the mechanism of triterpenes biosynthesis.
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