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[HPLC fingerprints of Cortex Moutan Carbonisatum]
Peng Li1, Xuelong Zhao, Li Zhang
1Key Laboratory for Modern Research of Traditional Chinese Medical Formulae of Jiangsu, Nanjing University of Traditional Chinese Medicine, Nanjing 210046 China.
Objective:
To establish the analytical method of the HPLC fingerprint of Cortex Moutan Carbonisatum and offer the evidence for quality control and quality evaluation of carbonized Cortex Moutan accordingly.
Method:
Ten batches of Cortex Moutan Carbonisatum were measured by HPLC with Paeonol as a reference substance and the HPLC analysis was performed on a Hedera ODS-3 Chromatographic Column (4.6 mm x 150 mm, 5 microm), with methanol to Acetonitrile (1:1) and 0.5% trifluoroacetic acid solution as mobile phase in gradient mode and the detection wavelength was at 258 nm.
Result:
The common mode of the HPLC fingerprints were set up. There were 11 common peaks in the fingerprint of ten samples, and the similar degrees to the ten batches were between 0.830-0.991.
Conclusion:
The method is simple, accurate and have a good reaptability. And the quality of Cortex Moutan Carbonisatum can be controlled effectively by the HPLC fingerprint.
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