Related Experiment Video
Updated: Jun 17, 2026

A Multicenter MRI Protocol for the Evaluation and Quantification of Deep Vein Thrombosis
Published on: June 2, 2015
[Evaluation of five D-dimer assays for the exclusion of venous thromboembolism]
Jing Zhou1, Jia-Yu Sun, Jia-Guan Sun
1Clinical Laboratory Medical Department, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To evaluate the diagnostic performance of five D-dimer assays for the exclusion of suspected venous thromboembolis (VTE).
Methods:
The plasma D-dimer levels of 75 suspected VTE patients were tested with ELISA, VIDAS DD, DD Plus, Liatest STAGO DD, and NycoCard DD. The results of the five assays were compared with the gold standard of diagnosis of VTE.
Results:
Liatest STAGO DD, DD Plus, VIDAS DD and ELISA had good diagnostic performance for the exclusion of suspected VTE. Liatest STAGO DD had the greatest accuracy for differentiating diagnosis, with 0.814 of area under the curve (AUC), 96.7% of sensitivity, and 95.5% of negative predictive value. ELISA had the greatest specificity (48.9%). The five assays showed good correlations, with VIDAS DD and Liatest STAGO DD having the greatest correlation coefficient (r = 0.9106). VIDAS DD and Liatest STAGO DD correlated well with ELISA (r = 0.8764 and r = 0.8360 respectively).
Conclusion:
Liatest STAGO DD, DD Plus, and VIDAS DD have good diagnostic performance for the exclusion of VTE. They are easier and quicker to perform and can serve as an alternative test for ELISA. Further validation is needed for clinical applications.
Related Concept Videos
Venous Thrombosis II: Clinical Manifestations and Diagnostic Studies
Venous Thrombosis III: Interprofessional Care
Assessment of the Cardiovascular System III: Palpation
Jugular Venous Pressure (JVP) Measurement
Position the patient at a thirty- to forty-five-degree angle or in a semi-fowler's position. Look for the highest point of pulsation in the internal jugular vein and measure the vertical distance to the angle of Loius or sternal angle. A normal JVP is 3-4 cm above the...
Venous Thrombosis I: Introduction
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...
Venous Thrombosis IV: Nursing Management
