Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Infertility in Males01:23

Infertility in Males

Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...
Sperm Transport01:15

Sperm Transport

The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
Infertility in Females01:28

Infertility in Females

Female infertility is defined as the inability to conceive after a year of regular, unprotected intercourse and affects about 10–15% of couples worldwide. The primary cause of female infertility is ovulatory disorders, which hinder the release of eggs. These disorders can be classified as hypothalamic amenorrhea, polycystic ovarian syndrome (PCOS), premature ovarian failure, and hyperprolactinemic anovulation disorders.
Endometriosis, a condition characterized by abnormal growth of endometrial...
Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
Accessory Ducts of the Male Reproductive System01:25

Accessory Ducts of the Male Reproductive System

The male reproductive system is a complex network of organs and tissues that work together to produce and transport sperm. The epididymis, vasa deferens, ejaculatory ducts, and urethra are the accessory ducts involved in sperm maturation and transportation. These ducts play a critical role in the production and transportation of sperm from the testes to the urethra, where it is then released during ejaculation.
The epididymis is a small, comma-shaped organ located at the back of each testicle.
Accessory Glands of the Male Reproductive System01:16

Accessory Glands of the Male Reproductive System

The accessory ducts involved in sperm maturation and transportation include the epididymides, vasa deferentia, ejaculatory ducts, and urethra. These ducts play a critical role in the maturation, storage, and transportation of sperm from the testes to the urethra, where it is then released during ejaculation.
The epididymis is a small, comma-shaped organ located at the back of each testicle. The epididymis can be divided into three main parts: the head, body, and tail. The head of the epididymis...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Congenital bilateral absence of the vas deferens: the impact of spermatogenesis quality on intracytoplasmic sperm injection outcomes in 108 men.

Andrology·2015
Same author

Light microscopy morphological characteristics of the sperm flagellum may be related to axonemal abnormalities.

Andrologia·2014
Same author

Transition protein 1 mRNA expression is not related to pregnancy rate in azoospermic men undergoing TESE-ICSI.

Andrologia·2007
Same author

Optimal management of extreme oligozoospermia by an appropriate cryopreservation programme.

Human reproduction (Oxford, England)·2007
Same author

Pregnancies in cryptozoospermia with sperm ejaculated one day before ICSI: four case reports.

Journal of andrology·2006
Same author

[Study on psychiatric disorders and defensive process assessed by the "defense style questionnaire" in sterile males SAMPLE consulting in andrology].

L'Encephale·2006
Same journal

Fundamental research on the treatment of periodontitis with Kangfuxin (KFX) extended-release gel.

Folia histochemica et cytobiologica·2026
Same journal

Tectochrysin suppresses the profibrotic phenotype of human keloid fibroblasts and is associated with reduced nuclear p65 accumulation.

Folia histochemica et cytobiologica·2026
Same journal

IGFL2-AS1 knockdown enhances erastin-induced ferroptosis-associated changes in oral squamous cell carcinoma.

Folia histochemica et cytobiologica·2026
Same journal

Esculetin ameliorates sevoflurane-induced cognitive dysfunction in aged mice by suppressing neuroinflammation via inhibition of NF-κB/NLRP3 signaling.

Folia histochemica et cytobiologica·2026
Same journal

Lactucin inhibits growth and induces apoptosis in gastric cancer cells by targeting MAPK signaling.

Folia histochemica et cytobiologica·2026
Same journal

Inhibition of miR-33b-5p promotes osteoblast differentiation by regulating ABCA1 in cellular and animal models of osteoporosis.

Folia histochemica et cytobiologica·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2026

Vessel-Sparing Microsurgical Longitudinal Intussusception Vasoepididymostomy to Treat Epididymal Obstructive Azoospermia
06:28

Vessel-Sparing Microsurgical Longitudinal Intussusception Vasoepididymostomy to Treat Epididymal Obstructive Azoospermia

Published on: May 27, 2022

Secretory azoospermia or non-obstructive azoospermia?

J M Rigot1

  • 1Service d'Andrologie - CHRU de Lille, France.

Folia Histochemica Et Cytobiologica
|January 14, 2010
PubMed
Summary
This summary is machine-generated.

Surgical sperm retrieval via biopsy is effective for non-obstructive azoospermia, yielding a 50% sperm retrieval rate. This approach offers a viable option for male infertility treatment, with successful pregnancy and delivery rates observed.

More Related Videos

Clinical Application of 24 G Cannula Needle and 3-0 Polypropylene Suture in Vas Deferens Exploration
07:21

Clinical Application of 24 G Cannula Needle and 3-0 Polypropylene Suture in Vas Deferens Exploration

Published on: February 10, 2023

A Modified Vessel-Sparing Microsurgical Vasoepididymostomy
04:59

A Modified Vessel-Sparing Microsurgical Vasoepididymostomy

Published on: June 8, 2022

Related Experiment Videos

Last Updated: Jun 17, 2026

Vessel-Sparing Microsurgical Longitudinal Intussusception Vasoepididymostomy to Treat Epididymal Obstructive Azoospermia
06:28

Vessel-Sparing Microsurgical Longitudinal Intussusception Vasoepididymostomy to Treat Epididymal Obstructive Azoospermia

Published on: May 27, 2022

Clinical Application of 24 G Cannula Needle and 3-0 Polypropylene Suture in Vas Deferens Exploration
07:21

Clinical Application of 24 G Cannula Needle and 3-0 Polypropylene Suture in Vas Deferens Exploration

Published on: February 10, 2023

A Modified Vessel-Sparing Microsurgical Vasoepididymostomy
04:59

A Modified Vessel-Sparing Microsurgical Vasoepididymostomy

Published on: June 8, 2022

Area of Science:

  • Reproductive Medicine
  • Urology
  • Andrology

Background:

  • Azoospermia, the absence of sperm in ejaculate, is classified as obstructive or non-obstructive.
  • Non-obstructive azoospermia (NOA) presents a significant challenge in male infertility, requiring specialized diagnostic and therapeutic approaches.
  • Distinguishing between non-obstructive and secretory azoospermia is crucial for effective patient management.

Purpose of the Study:

  • To define the optimal management strategy for patients with non-obstructive azoospermia.
  • To evaluate the efficacy of testicular biopsy as a diagnostic and therapeutic tool in NOA.
  • To assess the outcomes of sperm retrieval and subsequent assisted reproductive technology in NOA patients.

Main Methods:

  • Comprehensive patient assessment including hormonal profiles and testicular volume.
  • Testicular sperm extraction (TESE) via biopsy as the primary method for sperm retrieval in NOA.
  • Analysis of sperm retrieval rates, intracytoplasmic sperm injection (ICSI) outcomes, pregnancy rates, and delivery rates.

Main Results:

  • Testicular biopsy is identified as the gold standard for sperm retrieval in non-obstructive azoospermia.
  • A mean sperm retrieval rate of 50% was achieved through surgical sampling.
  • Pregnancy and delivery rates per cycle were 20% and 30%, respectively, with improved success using cryopreserved sperm samples.

Conclusions:

  • Testicular biopsy is a highly effective "gold standard" procedure for sperm retrieval in non-obstructive azoospermia.
  • Optimizing residual spermatogenesis and considering female partner age are vital for successful outcomes.
  • Cryopreservation of retrieved sperm samples enhances the success rates of assisted reproduction in NOA cases.