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Monocyte expression of adhesion molecules during low- and high-flux polysulfone hemodialysis and the effect of

Aristeidis Stavroulopoulos1, Dimitrios Petras, Ioannis Kakavas

  • 1Nephrology Department, IASIO Hospital - General Clinic of Kallithea, Athens, Greece. stavroulopoulos@yahoo.co.uk

Blood Purification
|January 14, 2010
PubMed

Insights

Atorvastatin reduces monocyte activation during hemodialysis, regardless of membrane flux. Polysulfone dialysis itself activates monocytes, increasing CD11b/CD18 and decreasing CD62L expression.

Area of Science:

  • Nephrology
  • Immunology
  • Pharmacology

Background:

  • Hemodialysis using polysulfone membranes can activate monocytes.
  • Membrane flux (low-flux vs. high-flux) may influence this activation.
  • Adhesion molecules on monocytes are key indicators of activation.

Purpose of the Study:

  • To investigate the effect of atorvastatin on monocyte adhesion molecule expression.
  • To determine if membrane flux influences monocyte activation during hemodialysis.

Main Methods:

  • Studied CD11b, CD18, and CD62L expression on monocytes via flow cytometry.
  • 32 hemodialysis patients were analyzed: 16 on low-flux (LFD) and 16 on high-flux (HFD) polysulfone membranes.
  • Measurements were taken before and after dialysis, and after 6 months of atorvastatin (20 mg/day) administration.

Main Results:

  • Hemodialysis increased CD11b and CD18 expression while decreasing CD62L expression on monocytes.
  • Atorvastatin administration reduced pre-dialysis monocyte CD11b and CD18 expression and increased CD62L expression.
  • No significant differences in adhesion molecule expression were observed between LFD and HFD groups.

Conclusions:

  • The polysulfone dialysis process activates monocytes irrespective of membrane flux.
  • Atorvastatin treatment is associated with a reduction in monocyte activation markers.
Abstract

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