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Updated: Jun 17, 2026

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Bacterial and eukaryotic phosphoketolases: phylogeny, distribution and evolution.

Borja Sánchez¹, Manuel Zúñiga, Fernando González-Candelas

¹Université de Bordeaux, UMR 5248 CNRS, UBX1-ENITAB, ENITAB, Gradignan, France. borja@ipla.csic.es

Journal of Molecular Microbiology and Biotechnology

|January 14, 2010

Summary

Phosphoketolases (XFPs) are key glycolytic enzymes found across Eukarya and Bacteria. Their distribution and phylogenetic analysis strongly suggest horizontal gene transfer shaped their evolution.

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Last Updated: Jun 17, 2026

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

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Published on: April 29, 2022

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

Area of Science:

Biochemistry
Evolutionary Biology
Genomics

Background:

Phosphoketolases (XFPs) are crucial enzymes in glycolysis, present in diverse organisms across Eukarya and Bacteria.
Understanding the evolutionary history and distribution of XFPs provides insights into metabolic pathway evolution.

Purpose of the Study:

To investigate the distribution and evolutionary patterns of phosphoketolase (xfp) genes across various microbial taxa.
To determine the role of horizontal gene transfer in the evolution of XFPs.

Main Methods:

Bioinformatic analysis of 151 putative xfp genes from 650 public genomes.
Phylogenetic analysis of a curated dataset of 128 XFP sequences.
Examination of xfp gene distribution across different taxonomic groups and genome locations (chromosomal vs. plasmid).

Main Results:

A total of 128 high-quality phosphoketolase (xfp) genes were identified and analyzed.
Xfp gene distribution was uneven, with notable prevalence in Lactobacillaceae, Rhizobiales, and Actinobacteria.
Phylogenetic analyses revealed discordance with organismal descent, indicating xenologs rather than paralogs, and identified plasmid-borne xfp genes.

Conclusions:

Horizontal gene transfer is a significant factor in the evolutionary diversification of phosphoketolases.
The distribution patterns and phylogenetic incongruities of xfp genes support extensive horizontal gene transfer events.