Related Experiment Video
Updated: Jun 17, 2026

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition
Published on: August 20, 2016
The in vitro study of pressure change effect on corneal birefringence
Zhen Xiao1, Chong Wang, Yan-Sheng Hao
1The Peking University Third Hospital, Peking University Eye Center, Beijing, China.
Purpose:
To study the change of corneal birefringence at different pressures in vitro to determine whether corneal birefringence changes along with pressure.
Methods:
The pattern of corneal birefringence was recorded at different intraocular pressures. The pressure differences were 0.10, 0.50 and 1.00 mm Hg, respectively. To compare the pattern sequence at two pressure values, a nonpaired t test was performed.
Results:
Statistical analysis indicates that the difference of the corneal birefringence is significant when the pressure difference was more than 0.50 mm Hg in polarized light.
Conclusion:
The results of corneal photoelasticity in vitro demonstrate that the change in pressure on the cornea leads to a change in corneal birefringence.
