Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Interference and Diffraction02:18

Interference and Diffraction

Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Light Acquisition02:16

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Focusing of Light in the Eye01:16

Focusing of Light in the Eye

Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Broad-source image plane holography as a confocal imaging process.

Applied optics·2010
Same author

Superresolution by spatial-temporal encoding methods.

Applied optics·2010
Same author

Edge-illuminated holograms.

Applied optics·2010
Same author

Three-dimensional confocal imaging of objects embedded within thick diffusing media.

Applied optics·2010
Same author

Grating interferometers for producing large holographic gratings.

Applied optics·2010
Same author

Multiple Fourier transform generation for coherent optical correlators.

Applied optics·2010
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Imagery with pseudo-randomly diffused coherent illumination.

E N Leith, J Upatnieks

    Applied Optics
    |January 14, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Researchers improved coherent light image formation using a novel pseudorandom coded diffuser. This specially designed diffuser enhances image quality compared to traditional methods.

    More Related Videos

    Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging
    11:07

    Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging

    Published on: November 24, 2021

    Related Experiment Videos

    Last Updated: Jun 17, 2026

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging
    11:07

    Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging

    Published on: November 24, 2021

    Area of Science:

    • Optics and Photonics
    • Image Processing

    Background:

    • Coherent imaging techniques are susceptible to noise and aberrations.
    • Traditional diffusers like ground glass offer limited control over light scattering properties.

    Purpose of the Study:

    • To investigate the efficacy of pseudorandom coded diffusers for enhancing coherent image formation.
    • To explore a novel method for improving image quality in coherent optical systems.

    Main Methods:

    • A pseudorandom coded diffuser was designed with specific constraints.
    • Coherent light beams were passed through this diffuser.
    • Image formation quality was analyzed and compared to conventional methods.

    Main Results:

    • The pseudorandom coded diffuser demonstrated improved image formation characteristics.
    • Enhanced control over light scattering led to reduced noise and improved resolution.
    • The diffuser's performance surpassed that of standard ground glass.

    Conclusions:

    • Pseudorandom coded diffusers offer a promising approach to enhance coherent imaging.
    • This method provides a viable solution for improving image quality in demanding optical applications.