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Related Experiment Video

Updated: Jun 17, 2026

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

Blood-brain barrier defects associated with Rbp9 mutation.

Jihyun Kim¹, Young-Joon Kim, Jeongsil Kim-Ha

¹Department of Molecular Biology, College of Life Sciences, Sejong University, Seoul, 143-747, Korea.

Molecules and Cells

|January 14, 2010

Summary

RNA-binding protein Rbp9 is crucial for Drosophila lifespan and locomotion. Mutants exhibit reduced activity and shortened life, linked to impaired blood-brain barrier function and cell adhesion molecule regulation.

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Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

Rbp9 is a Drosophila RNA-binding protein homologous to Elav and human Hu proteins.
Elav mutations cause CNS defects, and Hu is linked to neurological disorders.

Purpose of the Study:

To investigate the role of Rbp9 in Drosophila, particularly concerning neurological function and lifespan.
To understand the molecular mechanisms behind Rbp9's function.

Main Methods:

Generation of Rbp9 mutant flies.
Analysis of mutant phenotypes including locomotor activity and lifespan.
Gene expression profiling and characterization of potential target genes.

Main Results:

Rbp9 mutants displayed normal development but reduced locomotor activity and halved lifespan.
Gene expression analysis revealed reduced cell adhesion molecules and blood-brain barrier defects.
Rbp9-binding sites were identified in introns of cell adhesion molecule genes.

Conclusions:

Rbp9 plays a vital role in maintaining adult fly health, affecting locomotion and lifespan.
Rbp9 likely regulates cell adhesion molecule splicing, essential for blood-brain barrier integrity.