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Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
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Published on: January 7, 2019

Transcriptional target-based expression cloning of immunoregulatory molecules

Rebecca L Lamason1, Stefanie M Lew, Joel L Pomerantz

  • 1Department of Biological Chemistry, Institute for Cell Engineering, and Graduate Program in Immunology, The Johns Hopkins University School of Medicine, BRB 607, Baltimore, MD 21205, USA.

Immunologic Research
|January 14, 2010
PubMed

Immunologic research has benefited tremendously from expression-cloning strategies designed to isolate genes responsible for a wide variety of immunomodulatory activities, including cytokines, receptors, signaling proteins, and transcription factors. Here, we discuss the use of expression-cloning strategies that have been modified to detect cDNAs that influence gene expression as assayed by a transcriptional reporter. We summarize our experience with these screens, review important parameters, and discuss potential modifications.

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