Related Experiment Video
Updated: Jun 17, 2026

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
Published on: January 27, 2016
Mapping networks of protein-mediated physical interactions between chromatin elements
Vijay K Tiwari1, Stephen B Baylin
1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
Combined 3C-ChIP-Cloning (6C) technology identifies proteins mediating chromatin interactions. This method maps interactions and protein roles in nuclear organization, advancing transcriptional regulation understanding.
Area of Science:
- Molecular Biology
- Genomics
- Epigenetics
Background:
- Transcriptional regulation understanding advanced with new technologies determining chromatin proximities.
- Existing methods lack specificity in identifying protein mediators of chromatin interactions.
Purpose of the Study:
- To provide a detailed protocol for Combined 3C-ChIP-Cloning (6C) technology.
- To enable simultaneous identification of chromatin interaction proximities and mediating proteins.
- To explore combining 6C with other techniques for comprehensive, cell-type-specific mapping of protein-mediated interactions.
Main Methods:
- Detailed protocol for Combined 3C-ChIP-Cloning (6C) technology.
- Integration of 6C assay with other techniques for advanced mapping.
Main Results:
- 6C technology successfully identifies physical proximities between chromatin elements.
- 6C technology identifies the specific proteins mediating these chromatin interactions.
- The 6C assay facilitates cell-type-specific mapping of inter- and intrachromosomal interactions.
Conclusions:
- The 6C assay is a valuable tool for studying nuclear organization.
- 6C technology advances understanding of the relationship between chromatin higher-order organization and transcriptional regulation.
- This method elucidates the role of specific proteins in nuclear organization and gene regulation.
More Related Videos
21:55Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation
Published on: April 30, 2012
24:02The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
Published on: April 11, 2014
Related Concept Videos
Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Spreading of Chromatin Modifications
Writers
The writer is an enzyme that can...
Nucleosome Remodeling
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Chromatin Immunoprecipitation- ChIP
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Chromatin Position Affects Gene Expression
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...