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[Determination of pseudoprodioscin in Dioscorea panthaica by HPLC]
Wenguang Jing1, Qiwei Zhang, An Liu
1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Objective:
To develop an HPLC method for the determination of pseudoprodioscin in Dioscorea panthaica and provide scientific basis for quality control of it and its preparation.
Method:
The analysis was carried out on a Merck Purospher STAR RP-18e column eluted with acetonitrile and water as mobile phases in gradient mode. The detection wavelength was 203 nm, and the flow rate was 1.0 mL min(-1).
Result:
The pseudoprodioscin in D. panthaica extract was well separated. The linear range is between 0.0800-4.00 microg, r =0.9999. The average recovery and RSD are 99.9% and 2.3%, respectively.
Conclusion:
This method for quantitation of pseudoprodioscin in D. panthaica is simple, accurate and reliable and can be used for the quality control of D. panthaica and its preparations.
