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Avian tumour virus interactions with chicken fibroblast membranes: partial characterization of initial attachment

C F Moldow, P Volberding, M McGrath

    The Journal of General Virology
    |November 1, 1977
    PubMed

    Insights

    Trypsin solubilizes avian tumor virus (ATV) attachment sites on chicken embryo fibroblasts (CEF). Soluble sites bind ATV, reducing viral infectivity and transforming capacity in vitro.

    Area of Science:

    • Virology
    • Cell Biology
    • Biochemistry

    Background:

    • Avian tumor viruses (ATVs) require specific attachment sites on host cells for infection.
    • Chicken embryo fibroblasts (CEFs) are a model system for studying viral interactions.
    • Cell surface components can be released by enzymatic treatment.

    Purpose of the Study:

    • To investigate the effect of trypsin treatment on CEF cell surface components.
    • To determine if solubilized CEF components interact with ATVs.
    • To assess the impact of this interaction on ATV infectivity.

    Main Methods:

    • Exposure of CEF to trypsin to solubilize cell surface proteins.
    • In vitro assays to study the interaction between solubilized CEF components and ATVs.
    • Measurement of ATV infectivity and transforming capacity (e.g., RSV).

    Main Results:

    • Trypsin treatment releases ATV attachment sites from CEF.
    • Soluble attachment sites directly interact with ATV in vitro.
    • This interaction inhibits ATV binding to CEF and reduces viral infectivity and transforming capacity.

    Conclusions:

    • Soluble cell surface attachment sites can neutralize ATV infectivity.
    • This finding provides insights into viral entry mechanisms and potential therapeutic targets.
    • The study demonstrates a method to interfere with ATV infection using solubilized host factors.

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