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Related Experiment Video

Updated: Jun 17, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

Tipping the balance by manipulating post-translational modifications.

Christopher A Broberg¹, Kim Orth

¹Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9148, USA.

Current Opinion in Microbiology

|January 15, 2010

Summary

This summary is machine-generated.

Bacteria deliver virulence factors using type III secretion systems to manipulate host defenses. These bacterial effectors utilize post-translational modifications to control host signaling and promote infection.

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Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

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Last Updated: Jun 17, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

Area of Science:

Microbiology
Cellular Biology
Molecular Biology

Background:

Bacteria employ sophisticated mechanisms to infect host cells.
Type III secretion systems (T3SS) are crucial for delivering bacterial virulence factors directly into host cells.
These delivered factors often mimic or interfere with host cellular processes.

Purpose of the Study:

To characterize a novel class of bacterial effector proteins delivered by T3SS.
To investigate the role of post-translational modifications in effector function.
To understand how these effectors manipulate host signaling pathways.

Main Methods:

Analysis of bacterial effector proteins.
Investigation of post-translational modifications (PTMs).
Study of host-pathogen interactions and signaling pathways.

Main Results:

Identified a class of T3SS effectors utilizing post-translational modifications.
Demonstrated both reversible and irreversible PTMs are employed by these effectors.
Showcased the manipulation of host signaling pathways by bacterial effectors.

Conclusions:

Bacterial effectors use diverse post-translational modifications to subvert host responses.
Understanding these mechanisms is key to combating bacterial infections.
This highlights a conserved strategy used by pathogens to ensure survival.